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22 octubre 2014 3 22 /10 /octubre /2014 16:10


A paralysed man has been able to walk again after a pioneering therapy that involved transplanting cells from his nasal cavity into his spinal cord.

Cell transplant helps paralyzed man walk with frame | Reuters




Spinal graphic


1) Uno de los dos bulbos olfatorios del paciente se retiró y las células de la glia envolvente olfatoria (células olfativas de Scwann o OECO olfactory ensheathing cells) se cultivaron en laboratorio
2) 100 micro inyecciones de las OECs se realizaron por encima y por debajo de la zona dañada de la médula espinal
3) Cuatro tiras de tejido nervioso se colocaron a través de una brecha de 8 mm en la médula espinal. Los científicos creen que  las OECs estimularon la regeneración de las células de la médula espinal, utilizando los injertos de nervios tomados del tobillo del paciente como un puente para cruzar la médula seccionada 


Darek undergoing physiotherapy  
Mr Fidyka undergoes five hours of physiotherapy a day


Generic image of a person smelling

The complex neural circuitry responsible for our sense of smell is the only part of the nervous system that regenerates throughout adult life.

El complejo circuito neural responsable de nuestro sentido del olfato es la única parte del sistema nervioso que se regenera durante la vida adulta. Esto ha sido aprovechado para hace caminar a un paralítico


Trasplante de células del olfato hace caminar a paralítico

22 de octubre de 2014 Periódico La Jornada
Londres, martes 21 de octubre.

Un exitoso trasplante de células extraídas de la cavidad nasal a la médula espinal permitió a un paralítico volver a caminar tras permanecer inmóvil a causa de lesiones sufridas en la espalda hace cuatro años.

Se trata del primer procedimiento quirúrgico de este tipo, realizado por cirujanos de Polonia en colaboración con científicos británico. Sus resultados son una primicia mundial.

Darek Fidyka, de 40 años, quedó paralizado en 2010 tras ser agredido en la espalda con una arma blanca que le perforó la médula espinal, pero gracias al trasplante y la rehabilitación física posoperatoria pudo ponerse en pie y caminar, con ayuda de una andadera.

Para lograrlo se utilizaron células especiales que son parte de olfato, las cuales permiten la renovación continua de las fibras nerviosas en ese sistema, explican los especialistas en su informe, publicado en la revista Cell Transplantation.

Se realizaron dos operaciones; en la primera, los cirujanos quitaron uno de los bulbos olfativos del paciente e hicieron crecer las células en cultivo.

Dos semanas después trasplantaron las células –alrededor de 500 mil– en la médula espinal dañada, con cerca de 100 microinyecciones encima y por debajo de la lesión, donde posteriormente colocaron cuatro tiras delgadas de tejido nervioso tomadas del tobillo del paciente.

Los científicos indican que las células proporcionaron una vía que permitió la renovación de fibras por encima y debajo de la lesión y con los injertos se reconstruyó la médula.

Antes del tratamiento, Fidyka había estado paralizado casi dos años, sin señales de recuperación a pesar de muchos meses de fisioterapia intensiva








Paralysed man walks again after cell transplant


A paralysed man has been able to walk again after a pioneering therapy that involved transplanting cells from his nasal cavity into his spinal cord.

Darek Fidyka, who was paralysed from the chest down in a knife attack in 2010, can now walk using a frame.

The treatment, a world first, was carried out by surgeons in Poland in collaboration with scientists in London.

Details of the research are published in the journal Cell Transplantation.

BBC One's Panorama programme had unique access to the project and spent a year charting the patient's rehabilitation.

Darek Fidyka, 40, from Poland, was paralysed after being stabbed repeatedly in the back in the 2010 attack.

He said walking again - with the support of a frame - was "an incredible feeling", adding: "When you can't feel almost half your body, you are helpless, but when it starts coming back it's like you were born again."

Prof Geoff Raisman, chair of neural regeneration at University College London's Institute of Neurology, led the UK research team. He said what had been achieved was "more impressive than man walking on the moon".

The treatment used olfactory ensheathing cells (OECs) - specialist cells that form part of the sense of smell.
OECs act as pathway cells that enable nerve fibres in the olfactory system to be continually renewed.

In the first of two operations, surgeons removed one of the patient's olfactory bulbs and grew the cells in culture.
Two weeks later they transplanted the OECs into the spinal cord, which had been cut through in the knife attack apart from a thin strip of scar tissue on the right. They had just a drop of material to work with - about 500,000 cells.

About 100 micro-injections of OECs were made above and below the injury.
Four thin strips of nerve tissue were taken from the patient's ankle and placed across an 8mm (0.3in) gap on the left side of the cord.
The scientists believe the OECs provided a pathway to enable fibres above and below the injury to reconnect, using the nerve grafts to bridge the gap in the cord.



Spinal graphic

1) One of the patient's two olfactory bulbs was removed and the olfactory ensheathing cells (OECs) were grown in culture

2) 100 micro injections of OECs were made above and below the damaged area of the spinal cord

3) Four strips of nerve tissue were placed across an 8mm gap in the spinal cord. The scientists believe the OECs acted as a pathway to stimulate the spinal cord cells to regenerate, using the nerve grafts as a bridge to cross the severed cord




Before the treatment, Mr Fidyka had been paralysed for nearly two years and had shown no sign of recovery despite many months of intensive physiotherapy.

This programme of exercise - five hours per day, five days a week - has continued after the transplant at the Akson Neuro-Rehabilitation Center in Wroclaw.

Mr Fidyka first noticed that the treatment had been successful after about three months, when his left thigh began putting on muscle.

Six months after surgery, Mr Fidyka was able to take his first tentative steps along parallel bars, using leg braces and the support of a physiotherapist.

Two years after the treatment, he can now walk outside the rehabilitation centre using a frame.

He has also recovered some bladder and bowel sensation and sexual function.

Dr Pawel Tabakow, consultant neurosurgeon at Wroclaw University Hospital, who led the Polish research team, said: "It's amazing to see how regeneration of the spinal cord, something that was thought impossible for many years, is becoming a reality."


Mr Fidyka still tires quickly when walking, but said: "I think it's realistic that one day I will become independent.

"What I have learned is that you must never give up but keep fighting, because some door will open in life."

The groundbreaking research was supported by the Nicholls Spinal Injury Foundation (NSIF) and the UK Stem Cell Foundation (UKSCF)

UKSCF was set up in 2007 to speed up progress of promising stem cell research - the charity has to date contributed £2.5m

NSIF was set up by chef David Nicholls after his son Daniel was paralysed from the arms down in a swimming accident in 2003.

To date the charity has given £1m to fund the research in London and a further £240,000 for the work in Poland.

The breakthrough

A key difference with Mr Fidyka was that the scientists were able use the patient's olfactory bulb, which is the richest source of olfactory ensheathing cells.

This meant there was no danger of rejection, so no need for immunosuppressive drugs used in conventional transplants.

Most of the repair of Mr Fidyka's spinal cord was done on the left side, where there was an 8mm gap.

He has since regained muscle mass and movement mostly on that side.

Scientists believe this is evidence that the recovery is due to regeneration, as signals from the brain controlling muscles in the left leg travel down the left side of the spinal cord.

MRI scans suggest that the gap in the cord has closed up following the treatment.

None of those involved in the research want to profit from it.

Prof Geoff Raisman said: "It would be my proudest boast if I could say that no patient had had to pay one penny for any of the information we have found."

NSIF said if there were any patents arising, it would acquire them so as to make the technique freely available.




The sense of smell and spinal repair


Generic image of a person smelling


The complex neural circuitry responsible for our sense of smell is the only part of the nervous system that regenerates throughout adult life.

It is this ability that scientists have tried to exploit in stimulating repair in the spinal cord.

Every time we breathe, molecules carrying different odours in the air come into contact with nerve cells in the nose.

These transmit messages to our olfactory bulbs - at the very top of the nasal cavity, sitting at the base of the brain.

The nerve cells are being continually damaged and must be replaced.

This process of regeneration is made possible by olfactory ensheathing cells (OECs), which provide a pathway for the fibres to grow back.




Mr Nicholls said: "When Dan had his accident I made him a promise that, one day, he would walk again. I set up the charity to raise funds purely for research into repairing the spinal cord. The results with Darek show we are making significant progress towards that goal."

Prof Wagih El Masri said: "Although the clinical neurological recovery is to date modest, this intervention has resulted in findings of compelling scientific significance."

The consultant spinal injuries surgeon, who has treated thousands of patients in the UK, added: "I have waited 40 years for something like this."

All those involved in the research are keen not to raise false hopes in patients and stress that the success will need to be repeated to show definitively whether it can stimulate spinal cord regeneration.

The scientists hope to treat another 10 patients, in Poland and Britain over the coming years, although that will depend on the research receiving funding.

Dr Tabakow said: "Our team in Poland would be prepared to consider patients from anywhere in the world who are suitable for this therapy. They are likely to have had a knife wound injury where the spinal cord has been cleanly severed.

Sir Richard Sykes, chair of the UK Stem Cell Foundation, said: "The first patient is an inspirational and important step, which brings years of laboratory research towards the clinical testbed."

"To fully develop future treatments that benefit the 3 million paralysed globally will need continued investment for wide scale clinical trials,"


The researchers

BBC undated handout video grab of Professor Geoffrey Raisman  


Prof Raisman has spent more than 40 years studying how to repair the spinal cord.

In animal studies he showed that OECs injected into the rat spinal cord could reverse paralysis.

In 2005, Prof Raisman was approached by a Polish neurosurgeon who had begun researching how to apply the technique in humans.


BBC undated handout video grab of Dr Pawel Tabakow  


Dr Tabakow carried out an initial trial involving three paralysed patients who each had a small amount of OECs injected in their damaged spinal cords.

While none showed any significant improvement, the main purpose of the study was achieved, showing that the treatment was safe.




[PDF]Células en Glia envolvente olfatoria - Facultad de Medicina

de R GÓMEZ - ‎2007 - ‎
Las células de la glía envolvente olfatoria hacen parte del tejido del sistema .... oligodendrocitos, células de Schwann ... olfatoria se derivan de células precur-.


* Malcolm Allison H  2014

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